A single-step purification of bothropstoxin-1
نویسندگان
چکیده
منابع مشابه
A single-step purification of bothropstoxin-1.
Bothrops venoms are complex mixtures of components with a wide range of biological activities. Among these substances, myotoxins have been investigated by several groups. Bothropstoxin-1 (Bthtx-1) is a phospholipase A2-like basic myotoxin from Bothrops jararacussu. The purification of this component involves two chromatographic steps. Although providing a pure material, the association of these...
متن کاملA single step purification for recombinant proteins
A 167 base pair DNA cassette has been constructed to facilitate the detection and purification of recombinunt proteins. This cassette, kfc, cncodcs three distinct peptide units: a phosphorylation site for (he CAMP-depcndeni protein base (PKA), called hemptidc, a factor Xa cleavage site, and a calmodulin-binding peptide. Expressed kfc fusio? proteins can be purified from bacterial lysates in one...
متن کاملSingle-step affinity purification for fungal proteomics.
A single-step protein affinity purification protocol using Aspergillus nidulans is described. Detailed protocols for cell breakage, affinity purification, and depending on the application, methods for protein release from affinity beads are provided. Examples defining the utility of the approaches, which should be widely applicable, are included.
متن کاملSingle-step purification of specific tRNAs by hydrophobic tagging.
Studies on translation frequently require large amounts of purified individual tRNAs. However, few purified tRNAs are commercially available. Individual tRNAs can be easily produced by in vitro transcription, but the lack of modifications of the tRNA transcripts may influence or impair their function [1,2]. The purification of specific tRNAs from total tRNA is a laborious process that requires ...
متن کاملSingle step purification of ovine luteinizing hormone by affinity chromatography.
Affinity chromatography has been applied to the problem of specifically removing luteinizing hormone from sera used to supplement tissue culture media. Horse or fetal calf sera, passed through a column of an anti-luteinizing hormone immunoglobulin fraction linked covalently to agarose, were specifically depleted in their content of luteinizing hormone as seen by radioimmunoassay and by their ef...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Brazilian Journal of Medical and Biological Research
سال: 1998
ISSN: 0100-879X
DOI: 10.1590/s0100-879x1998000900004